Daphnia are extremely small and range in size from 0. Students will clarify the cause and effect relationship between alcohol or caffeine and daphnia heart rate. Daphnia heart rate lab report essay example graduateway. We use cookies to provide you with a great user experience. Studying heart rate using daphniastudent laboratory kit. Environment cladocerans are common in almost all aquatic environments, with the exception of fastmoving streams and. We used daphnia magna bioassays to determine the lc and the effects on the heart of the cardioactive drugs50. Have them record observations, draw diagrams, and write questions they have in their journal. Heart rate of daphnia, students observe the heart rate of daphnia at different temperatures. Abstract in this project, water fleas daphnia magna, a semitransparent freshwater crustacean, are used to study the effects of caffeine on heart rate. They were maintained at room temperature in the medium in which they were shipped. A daphnia is a tiny crustacean related to shrimp that has a clear outside skeleton exoskeleton and jointed legs. In this experiment on the crustacean, daphnia, tudents must collect data on how daphnia respond to changes in their environment. Temperatureenhanced activity appeared to be supported by cellular atp synthesis.
Drew, freeman, jayda, and jasmine abstract daphnia lab in the experiment, the purpose of it all was to identify what happens to a daphnias heart rate when being treated with ethanol alcohol. This investigation starts with a guided procedure where students gather data on the heart rate of daphnia when the organism is exposed to 1% ethanol daphnia are tiny crustaceans that are visible with the naked eye, though a microscope or stereoscope will be needed to see their heart rate. In the process of designing experiments, collecting data, and writing scientific papers. This is what happened to the daphnia under the microscope the drug caused their heart rate to increase and the different concentrations caused the heart rate to increase at different levels. With increase in nicotine concentration, the heart rate will rise. Both 5%% and 10 %% ethanol cause rapid decreases in heart rate that are large in magnitude. Animals were fed daphnia growth food daily wards catalog number 88w5950.
Heart rate pharmacology of the water flea, daphnia kingdom. Determine and record the heart rate in beats per minute bpm. There are videos available on the internet that can be used to help students distinguish between the daphnia heart and gills. Introduction to daphnia biology ecology, epidemiology. Initial experiments focused on the effects of ethanol 1%, 5%, and 10% vv, nicotine 0. The normal heart rate of a daphnia magna 1552 words bartleby. Effect of temperature on waterflea daphnia magna core. Lab report of daphnia magna heart rate essaytalents. Daphnia heart rate experiment essay example graduateway. Students then locate the heart of the daphnia and determine the heart rate in beats per minute.
Make sure the daphnia has plenty of water in the dish. This laboratory experiment was done on daphnia magna, a type of fresh water flea, to observe how it responds to neurotransmitters and temperature changes. These data suggest there is an increase in heart rate when a daphnia magna is exposed to caffeine and a decrease in heart rate when it is in a solution of alcohol. Ethanol is a highly suitable agent to test in a teaching laboratory focused on heart rate in daphnia. The heart rate of a single daphnia in only pond water was recorded, as well as the heart rate of a single daphnia in a 6% ethanol solution. Design an experiment to test the effects of either caffeine, or alcohol on the heart beat rate in daphnia. A webcam above the eyepiece of the microscope to project an image of the slide onto a large screen may also help with counting. The more concentrated caffeine solution given to the daphnia, the higher their heart rate became, for example the 0% caffeine solution gave a heart rate of 249 bpm and the 0. The three caffeine treatments we chose contained 100, 200, and 300 mg of caffeine.
Introduction to the heart the heart is a muscular organ that pumps circulatory fluid. All three compounds were obtained from sigma aldrich st. A daphnia magna is a species of water fleas and can be located in the northern united sates against the coastline of the atlantic in. Daphnia s common name of water flea comes from its jumplike movement, which results from the beat of the large antennae used for swimming figure 1. Determine and record the heart rate for 10 seconds. In the second part of this lab, you will study the relationship between temperature and metabolic activity in an ectothermic animal. The premier journal for undergraduate publications in the neurosciences 2009 effects of melatonin and ethanol on the heart rate of daphnia magna bonnie kaas1,2, krithika krishnarao2, elizabeth marion1, larissa stuckey1, and rebecca kohn3 1department of neuroscience, 2department of biochemistry and molecular biology, 3department of biology. Jul 08, 2011 this video is a stepbystep guide to properly observing daphnia heart rates in different temperatures and with different chemicals.
Effect of nicotine on the heart rate of daphnia barbra. Includes detailed teacher notes with helpful lab hints, answers to worksheet questions, and a reproducible student. Blood cells are easily visible through the transparent body as they flow rapidly through the body cavity. Cladocera at 2 o to 12 o c above their habitat temperature 16 o c for 6 months increased the rate of their metabolic activity respiration and heartbeat rates. This allows for observation of their internal organs, making it easy to observe certain life functions in daphnia. Although very few scientific studies have been done on.
Although that is what is expected, other organisms tend not to reactive to certain chemicals. Heart beat rate was measured using daphnia heart rate kit. Different percentage rates of ethanol were added to the petri dish where the daphnia was located and the. The study dopamine synergizes with caffeine to increase the heart rate of daphnia done by aman kundu and gyanesh singh, investigates the effect of dopamine, caffeine and alcohol on daphnia s heart rate. Introduction the purpose of this lab was to determine the normal heart rate of a daphnia magna and decipher the different effects that various substances had on it. Comparing the means of the heart rate at 50% and 75% concentrations, the pvalue is 0. Acohol concentration % daphnia heart rate bpm need similar work done. Introduction the leaves and roots of kava are used for drinks and medicinal purposes. Design an experiment to test the effects of either caffeine, or alcohol on the heart beat rate in. Drew, freeman, jayda, and jasmine abstract daphnia lab in the experiment, the purpose of it all was to identify what happens to a daphnia s heart rate when being treated with ethanol alcohol. Daphnia mature in approximately a week and can have thousands of offspring. Because much of the biology has been studied over the past 250 years and is considered to be common knowledge, i give no references to specific studies in most cases. Name tutor course date effect of nicotine on the heart rate of daphnia introduction when any new chemicals is introduced to a living organism, there must be a reaction or a certain change.
The reported effects of caffeine on daphnia heart rate are contradictory. Heart rate pharmacology of the water flea, daphnia kingdom animalia. Study on the effect of alcoholic beverages on the heart rate of daphnia daphnia magna paradela, immanuel p. In the studying heart rate using daphnia animal behavior laboratory kit for biology and life science, daphnia are exposed to both a stimulant and depressant as well as varying environmental temperatures. In this experiment, i am going to determine the effect of different concentration of caffeine on the heart rate of daphnia. C, it beats about 200 times per minute, slowing down at lower temperatures. Students view the organisms under a compound microscope to observe the effect of these conditions on heart rate. Pdf melatonin, an endogenous hormone that may regulate circadian rhythms by. Methodsmaterials five solutions were formed by mixing 0 ml, 59. Study on the effect of alcoholic beverages on the heart rate. In conclusion the null hypothesis was disapproved as the lower concentration of caffeine produced a lower rise in heart rate than the 0. The outer carapace of a daphnia is transparent so all the internal organs, even the beating heart, can be seen. Daphnia received the name water flea due to their jerky swimming motions. One way that they differ from those crustaceans is that their carapace is transparent.
The normal heart rate of a daphnia magna 1552 words. How do you think daphnia acquires o2 and expels co2. A daphnia magna is a species of water fleas and can be located in the northern united sates against the coastline of the atlantic in rocky pools. Introduction to the heart the heart is a muscular organ that pumps circulatory fluid blood or hemolymph throughout the body. We also used these treatments to evaluate the immediate short term effects on the average heart rate of the d. Physiological changes in the heart rate of daphnia magna when exposed to stimulants caffeine and alcohol. Missing references missing your lab manual see the page 3, example of lab manual missing in text citations missing your lab manual in text citations. The adrenalin used in these experiments was prepared by parke.
Learn how to measure the heartbeat rate in a small crustacean, daphnia. Introduction in this experiment, the objective was to find out if caffeine would effect the heart rate of daphnia. Kava decreases the heart rate of daphnia anova science. Both 5%% and 10%% ethanol cause rapid decreases in heart rate that are large in magnitude. Study on the effect of alcoholic beverages on the heart. A microscope and stopwatch were used to measure the changes in the daphnias heart rate. Open ended section allows students to explore other factors that can affect the heart rate. In this lab, daphnia are exposed to both a stimulant and depressant as well as varying environmental temperatures. Background information on daphnia betadifferentiatie. Heart rate in daphnia at different temperatures reading temperature. A single daphnia was placed in a beaker containing test solution for 5 minutes.
View lab report daphnia lab report from biol 160 at framingham state college. Accuracy can be improved by coupling a video camera to the. Enter this heart rate in the data tables and compute the heart rate per minute by multiplying by 6. Lamoureux caffeines effect on daphnia s heart rate j2310 objectivesgoals the objective of the experiment is to determine whether caffeine in the form of monster energy drink affects a daphnia s heart rate. The concentration of the caffeine in the blood of daphnia will determine the heart rate of the daphnia. To do this, use the counter app on your ipad and have your partner time you for 10 seconds. Daphnia can be found in freshwater lakes, ponds, and rivers all over the world. Click order now and fill your assignment details up to 15% discount offer.
Jan, 2010 daphnia are filter feeders that direct small suspended particles into their mouth by a water current produced by their leaflike legs figure 1. Use the following data for your lab results if you were not here or if you did not complete all of the required data collection in class. The average heart rate of daphnia under normal condition which acts as a control experiment is 114 heart beats per minute. Take at least three separate heart rate measurements for your daphnia and calculate the average of the three measurements.
Daphnia as possible refer to diagram of daphnia anatomy. Use a sphygmomanometer and stethoscope to measure systolic and diastolic blood pressure observe the response of the human circulatory system to various factors and use the results to determine the subjects level of physical fitness. When you have finished recording the heart rate in water the control solution, add one drop of your chosen. I feel that the same concept of the effect of alcohol and caffeine on daphnia magna would be the same concept for its effect on humans also sense alcohol is a depressant it tends to slow down. Science and math department, integrated developmental school, mindanao state university iligan institute of. Effects of melatonin and ethanol on the heart rate of daphnia magna. In this introductory physiology lab, students determine the heart rate of daphnia magna and then test the effect of changing temperature on the heart rate. Students will predict and test the effects of the etha nol on the heart rate of daphnia. Apr 12, 2016 this feature is not available right now. Introduction to daphnia biology ecology, epidemiology, and.
In this draft of the experiment to make it easy for students in future semester to repeat and obtain the same results. Daphnia lab report introduction in this experiment the. Study on the effect of alcoholic beverages on the heart rate of. Each experiment was repeated at least 5 times and the mean of the. The results show the heart rate of daphnia is positively affected by the presence of caffeine, and it is increased when the concentration of caffeine solution is raised.
We determined the effects of three concentrations of ethanol, nicotine, and caffeine and of a control solution on heart rate in daphnia. This figure shows a graphical representation of average heart rate of daphnia magna. There were three caffeine treatments and a spring water control 0 mg of caffeine. The heart is located dorsally and anterior from the brood chamber. Complete on your own write up this experiment as a formal lab report. The premier journal for undergraduate publications in the neurosciences 2009 effects of melatonin and ethanol on the heart rate of daphnia magna bonnie kaas1,2, krithika krishnarao2, elizabeth marion1, larissa stuckey1, and rebecca. In animals, an increase in cellular respiration triggers homeostatic mechanisms that increase both breathing and heart rate, resulting in more oxygen being available to cells.
How does the heart rate in daphnia compare to your own. Through the similarities daphnia and humans share, it is expected that nicotine will increase the heart rate of the daphnia immediately. If the three numbers are not within 10 % of each other, repeat your trials. The brain is the lightcolored organ lying above the eye. Also the combination of caffeine and ethanol was tested. Pdf effects of melatonin and ethanol on the heart rate of daphnia.
This study was the first to test the effects of kava on the heart rate of daphnia and suggests that it could have an effect on the health of humans. The water flea daphnia a new model system for ecology and. In that lab, you experimented with peas to see how the rate of oxygen consumption during cellular respiration increased with temperature. Acclimation of the waterflea daphnia magna straus crustacea. Jul 30, 2016 this investigation starts with a guided procedure where students gather data on the heart rate of daphnia when the organism is exposed to 1% ethanol daphnia are tiny crustaceans that are visible with the naked eye, though a microscope or stereoscope will be needed to see their heart rate. The results indicate that there is a distinct measurable change in heart rate when daphnia is immersed in a 0. The effects of drugs on daphnia van andel education institute. Daphnia s transparent body and large heart make it ideal for physiological studies.
Using videography to study the effects of stimulants on. Daphnia is assumed as the substitute of human being in this experiment to investigate the effect of chemicals on the heart beat of living organisms. At the highest concentration of alcohol, the heart rate slowed and in turn the higher concentration of caffeine affected the heart rate by increasing it. Various factors may be responsible for the reversal of phototactic reactions in daphnia. The effects of drugs on daphnia introduction daphnia, often called water fleas, are arthropods, related to crabs, lobsters, and other crustaceans. Then tests were conducted to determine its heart rate responses to neurotransmitters epinephrine and acetylcholine ach, low and room temperatures. Background information on daphnia part 1 physical description daphnia, commonly called water fleas, are a freshwater zooplankton found in ponds and lakes all over the world. Dopamine synergizes with caffeine to increase the heart rate. Summary conclusion that discusses the results of your experiment. The effects of alcohol on the heart rate of daphnia magna. Let us show you how to set up a popular heart rate measurement experiment using daphnia magna.
Repeat this procedure 2 more times and find the average heart rate of daphnia. The effect of alcohol on the heart rate of daphnia lab report the goal of this lab was to determine the effects that the presence of alcohol will have on the heart rate of a daphnia magna. It was hypothesized that the ethanol would decrease the heart rate of daphnia. The daphnia was examined and observed to document its heart rate hr. The american biology teacher making the most of the daphnia heart rate lab 177. It will be directly proportional to each other, meaning that the increase of the concentration of the caffeine will give an increase of the heartbeat. How do the various dilutions of nicotine affect heart rate. Daphnia lab report excellent essay writing services. Corotto and darrel ceballos and adam fraser lee and lindsey vinson, year2010. Lab 10 c heart rate and temperature the daphnia lab was conducted and the data that they got reported below. Students commonly test the effects of chemical agents on the heart rate of the crustacean daphnia magna, but the procedure has never been optimized.
Dont worry about having to learn how to take a crustaceans pulse. In this experiment, nicotines effect on the heart rate of a model specimen, daphnia magna is investigated. Record your quantitative and qualitative observations. In the cellular respiration laboratory, you experimented with peas and saw how the rate of oxygen consumption during cellular respiration varied with temperature. Its heart rate varies with water temperature, making it easy to alter the daphnia s heart rate and observe the changes. After the 5 minutes, the daphnia in a few drops of test solution was transferred to the slide for measurement of heart rate. This observation is true for all the alcoholic beverages used in the experiment. The following introduction summarizes the key aspects of the natural history of the genus daphnia. This experiment was done two times, and a paired t test analysis vs control indicate the following p. The effects of 4nonylphenol and ethanol on acute toxicity, embryo development, and reproduction in daphnia magna. Effect of temperature on waterflea daphnia magna crustacea. Take a sample daphnia and expose it to 1% ethanol and count the heart rate by observing the crustacean under a microscope. Hence, the null hypothesis is accepted and, therefore, there is no significant difference in the heart rate of daphnia exposed to the two concentrations. Have them do this as many times as they feel necessary.
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